Modifying Enzymes
Thermo Scientific™ Modifying Enzymes
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Invitrogen™ SUMO Protease
A recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae
Invitrogen™ Proteinase K, recombinant
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Invitrogen™ S1 Nuclease
Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides
Invitrogen™ Bacterial Alkaline Phosphatase
Removes 3' and 5' phosphates from DNA and RNA
R&D Systems™ Pan Caspase Inhibitor Z-VAD-FMK
Used as cell-permeable, irreversible inhibitor of caspase activity. R&D Systems Pan Caspase Inhibitor Z-VAD-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.
Invitrogen™ UltraPure™ Glycogen
Used as carrier for nucleic acid precipitation in ethanol
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix
Catalyzes in vitro recombination of PCR products to generate entry clones
Invitrogen™ ezDNase™ Enzyme
SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
Invitrogen™ Ribonuclease H
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA