Modifying Enzymes
Invitrogen™ AcTEV™ Protease
Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins
Corning™ Dispase
Bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on BD Matrigel™ Basement Membrane Matrix
Invitrogen™ T4 DNA Ligase Buffer
T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes
Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Invitrogen™ Proteinase K Solution
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.
Invitrogen™ UltraPure™ Glycogen
Used as carrier for nucleic acid precipitation in ethanol
Thermo Scientific™ Modifying Enzymes
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Invitrogen™ CorrectASE™ Enzyme
CorrectASE™ enzyme removes mismatches caused by oligonuceotide synthesis errors, leading to a 3–10 fold reduction in mutations in your synthetic genes or fragments.
R&D Systems™ Pan Caspase Inhibitor Z-VAD-FMK
Used as cell-permeable, irreversible inhibitor of caspase activity. R&D Systems Pan Caspase Inhibitor Z-VAD-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.
Invitrogen™ Proteinase K, recombinant
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.